含钙铁氧体磁性生物活性玻璃陶瓷热种子的制备与表征

Synthesis and Characterization of Magnetic Bioactive Glass Ceramic Thermoseeds Containing Ca Ferrite

中文关键词: 磁性生物活性玻璃陶瓷 热种子 钙铁氧体 磁热疗

英文关键词: magnetic bioactive glass ceramics, thermoseeds, Ca ferrite, magnetically induced hyperthermia

中文摘要:

      本工作将钙铁氧体与溶胶-凝胶法制得的生物活性玻璃陶瓷复合,制备了含钙铁氧体磁性生物活性玻璃陶瓷磁热种子。对所制得材料的物相组成、磁性、体外生物活性、磁生热能力及磁热对肿瘤细胞的杀伤效应、组织相容性进行了表征。结果表明,材料主晶相为CaSiO₃、Ca₅(PO₄)₃F和CaFe₂O₄。充磁约8×10⁵ A·m-1时,材料饱和磁化强度约5 A·m²·kg-1,矫顽力约2×10⁴ A·m-1。暴露在342 kHz、1×10³ A/m的交变磁场下,0.1 g材料20 min内即能实现升温约40 ℃。在浸泡入模拟体液中14 d后,材料表面能形成不连续的含有碳酸根生物的活性层。MTT细胞增殖实验显示材料无细胞毒性,MG63细胞能在材料上粘附和生长。将材料与VX2细胞在交变磁场下复合培养20 min,材料周围出现明显的死亡细胞圈。兔肌肉植入实验显示材料组织相容性良好。

英文摘要:

      In present research, magnetic bioactive glass ceramic thermoseed containing Ca ferrite was prepared using the bioa-ctive glass ceramic precursor synthesized by sol-gel method and Ca ferrite. Experiments were carried out to evaluate the crystalline composition, magnetic property, heat generating ability under alternative magnetic field, as well as the lethal effect of the magnetically induced heat to cells, bioactivity in vitro and tissue biocompability in vivo. The results revealed that the main crystal of the material was CaSiO₃, Ca₅(PO₄)₃F and CaFe₂O₄. Under a magnetic field about 8×10⁵ A·m-1, the saturation magnetization and coercive force of the composite were about 5 A·m²·kg-1 and 2×10⁴ A·m-1, respectively. When exposed to an alternative magnetic field with the frequency of 342 kHz and the magnitude of 1×10³ A/m, the material can realize a temperature rise of 40 ℃ in 20 min. After soaking in simulated body fluid for 14 d, a non-continuous biological active layer containing carbonate could be observed on the material surface. The MTT assay revealed that the material had no cytotoxicity. MG 63 cells could adhere and grow on the material. Moreover, the experiment of material and cell co-culturing under alternative magnetic filed showed that a circle of dead cell appeared around the material. The results of rabbit muscle implantation showed that the material had good tissue biocompatibility in vivo.